8EQ3
Escherichia coli pyruvate kinase A301T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2012-03-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.219, 247.074, 130.706 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.850 - 2.010 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yng |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.850 | 2.082 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.042 | 0.324 |
Number of reflections | 80594 | 7347 |
<I/σ(I)> | 13.63 | 1.8 |
Completeness [%] | 99.1 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M (Malonic acid, Imidazole, Boric acid), 25 % w/v PEG 1500 |