8EPI
Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 36.377, 36.377, 104.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.380 - 2.550 |
R-factor | 0.2511 |
Rwork | 0.249 |
R-free | 0.29110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ideal B type DNA duplexes generated in Cadnano |
RMSD bond length | 0.012 |
RMSD bond angle | 1.500 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.380 | 3.117 |
High resolution limit [Å] | 2.550 | 2.555 |
Number of reflections | 1308 | 188 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 80.4 | |
Redundancy | 10.8 | 10.1 |
CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.5 ug/uL motif 0.005 M magnesium sulfate hydrate, 0.005 M HEPES sodium pH 7.0, 0.16 M lithium sulfate monohydrate, 4 % MPD at pH 7.0 |