8EPD
Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2022-03-02 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 34.166, 34.166, 104.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.810 - 2.420 |
R-factor | 0.2488 |
Rwork | 0.246 |
R-free | 0.29810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ideal B type DNA duplexes generated in Cadnano |
RMSD bond length | 0.011 |
RMSD bond angle | 1.336 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.810 | 2.924 |
High resolution limit [Å] | 2.420 | 2.421 |
Number of reflections | 1864 | 189 |
<I/σ(I)> | 19.4 | |
Completeness [%] | 87.5 | |
Redundancy | 11.4 | 8.1 |
CC(1/2) | 1.000 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.5 ug/uL motif 0.005 M magnesium sulfate hydrate, 0.005 M HEPES sodium pH 7.0, 0.16 M lithium sulfate monohydrate, 4 % MPD at pH 7.0 |