8EOV

Precisely patterned nanofibers made from extendable protein multiplexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 32 2 1
Unit cell lengths	49.140, 49.140, 111.211
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.560 - 1.590
R-factor	0.205
Rwork	0.203
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Designed model
RMSD bond length	0.006
RMSD bond angle	0.623
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	1.640
High resolution limit [Å]	1.590	1.590
Rmerge	0.013	1.038
Number of reflections	21628	2120
<I/σ(I)>	23.39	0.68
Completeness [%]	99.8	99.67
Redundancy	2	2
CC(1/2)	1.000	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH 6.0, 3.2 M Ammonium sulfate