8EOM

TUDOR DOMAIN OF TUMOR SUPPRESSOR P53BP1 WITH MFP-5973

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.523, 51.188, 105.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.270 - 1.700
R-factor	0.1986
Rwork	0.197
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3r
RMSD bond length	0.011
RMSD bond angle	1.489
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.062	0.029	0.758
Rmeas	0.073	0.034	0.881
Rpim	0.036	0.017	0.441
Total number of observations	102459
Number of reflections	26188	1448	1287
<I/σ(I)>	8.5
Completeness [%]	98.4	97.7	98.2
Redundancy	3.9	3.9	3.8
CC(1/2)		0.998	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	2M ammonium sulfate, 0.1 M HEPES pH 7.5, 2% PEG 400