8EO8
Cross-reactive 3180 TCR recognition of HLA-B*35:01-NP8 epitope from 2005 H1N1 influenza strain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95365 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 168.088, 79.468, 172.173 |
Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
Resolution | 48.947 - 2.300 |
R-factor | 0.182718374785 |
Rwork | 0.181 |
R-free | 0.21859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lks |
RMSD bond length | 0.014 |
RMSD bond angle | 1.662 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.057 | 0.396 |
Number of reflections | 201262 | 20019 |
<I/σ(I)> | 13.1 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | PEG, ACETATE |