8EO0

Crystal structure of alpha-COPI WD40 domain R300A mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	37.940, 171.326, 71.583
Unit cell angles	90.00, 100.28, 90.00

Refinement procedure

Resolution	28.970 - 1.800
R-factor	0.1787
Rwork	0.177
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s22
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.970	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	82865	4564
<I/σ(I)>	4.6	1.1
Completeness [%]	100.0	99.9
Redundancy	4.7	4.7
CC(1/2)	0.991	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.2M Potassium sodium tartrate tetrahydrate, 18%PEG3350