8ENZ

Crystal structure of alpha-COPI-WD40 domain K15A mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	37.487, 172.265, 71.483
Unit cell angles	90.00, 99.91, 90.00

Refinement procedure

Resolution	28.710 - 1.650
R-factor	0.1645
Rwork	0.163
R-free	0.19340
RMSD bond length	0.014
RMSD bond angle	1.230
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.840	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	105146	10362
<I/σ(I)>	8.4	2.2
Completeness [%]	98.6	98.2
Redundancy	3.6	3.7
CC(1/2)	0.995	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.14M Sodium citrate tribasic dihydrate, 18% PEG3350