8ENW
Crystal structure of beta'-COPI-WD40 domain in complex with SARS-CoV-2 clientized spike tail heptapeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.711, 46.012, 84.319 |
| Unit cell angles | 81.24, 81.52, 69.01 |
Refinement procedure
| Resolution | 42.710 - 1.450 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.17960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j79 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.946 |
| Data reduction software | HKL-3000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.910 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 100965 | 4966 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 96.5 | 94.78 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.996 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.1M MES pH 6.0, 15% PEG20000 |
