8ENF

Crystal structure of LGR ligand alpha2/beta5 from C. elegans in crystal form 1 (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	89.733, 89.733, 99.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.870 - 3.290
R-factor	0.3056
Rwork	0.302
R-free	0.34430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8enb
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	XDS (2019-04-17)
Data scaling software	STARANISO
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.870	3.570
High resolution limit [Å]	3.290	3.290
Rmerge	0.112
Number of reflections	6059	404
<I/σ(I)>	14.1
Completeness [%]	93.2
Redundancy	19.7
CC(1/2)	0.999	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M magnesium chloride, 0.1 M MES, pH 6.0, 50% w/v PEG200