8EN8

Cross-reactive 3180 TCR recognition of HLA-B*35:01-NP4 epitope from 1972 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	79.770, 116.686, 253.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.196 - 2.700
R-factor	0.209126284475
Rwork	0.207
R-free	0.24852
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.012
RMSD bond angle	1.623
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.200	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.586
Rmeas	0.072
Number of reflections	65736	6466
<I/σ(I)>	13.9	2
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.993	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG