8EN7
Mouse apoferritin heavy chain without zinc determined using single-particle cryo-EM with Apollo camera.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON APOLLO (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.68 |
| Number of Particle | 183073 |
| Software | RELION,Leginon,CTFFIND |
| Symmetry | O |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
