8EN6

Structure of GII.4 norovirus in complex with Nanobody 76

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	74.412, 119.706, 127.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.310 - 1.600
R-factor	0.174
Rwork	0.173
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oos
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.370	87.370	1.560
High resolution limit [Å]	1.530	8.380	1.530
Rmerge	0.083	0.028	0.751
Rmeas	0.094	0.033	0.864
Rpim	0.044	0.015	0.419
Total number of observations	754982	5191	33211
Number of reflections	171712	1168	8330
<I/σ(I)>	9.3	27.6	1.5
Completeness [%]	99.8	98.4	98.4
Redundancy	4.4	4.4	4
CC(1/2)	0.996	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	17% [w/v] PEG4000, 0.0095 M HEPES [pH 7.5], 8.5% [v/v] isopropanol, and 15% glycerol