8EN4
Structure of GII.4 norovirus in complex with Nanobody 53
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 108.411, 137.292, 140.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.040 - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oos |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.100 | 54.100 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.900 | 2.300 |
| Rmerge | 0.067 | 0.035 | 0.432 |
| Rmeas | 0.075 | 0.040 | 0.482 |
| Rpim | 0.033 | 0.017 | 0.211 |
| Total number of observations | 236322 | 4197 | 22404 |
| Number of reflections | 46757 | 890 | 4469 |
| <I/σ(I)> | 11.8 | 25.3 | 3.1 |
| Completeness [%] | 99.7 | 99.2 | 98.3 |
| Redundancy | 5.1 | 4.7 | 5 |
| CC(1/2) | 0.998 | 0.998 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 1.6 M ammonium sulfate, 0.08 M sodium acetate, and 20% [v/v] glycerol |
