8EN2

Structure of GII.10 norovirus in complex with Nanobody 34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976200
Spacegroup name	P 1 21 1
Unit cell lengths	66.781, 80.528, 84.206
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	51.400 - 1.850
R-factor	0.2091
Rwork	0.208
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3onu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.528	80.528	1.960
High resolution limit [Å]	1.850	5.540	1.850
Rmerge	0.074	0.026	1.107
Rmeas	0.080	0.028	1.195
Total number of observations	533312
Number of reflections	75491	2899	12089
<I/σ(I)>	16.48	61.76	1.73
Completeness [%]	99.1	98.7	98.5
Redundancy	7.065	7.1	7.015
CC(1/2)	0.999	1.000	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	1 M lithium chloride, 10% [w/v] PEG6000, and 0.1 M citric acid [pH 5]