8EN1
Structure of GII.4 norovirus in complex with Nanobody 30
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97626 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 117.767, 62.443, 121.396 |
Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
Resolution | 57.750 - 2.400 |
R-factor | 0.2099 |
Rwork | 0.207 |
R-free | 0.26350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x7e |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.750 | 57.750 | 2.490 |
High resolution limit [Å] | 2.400 | 8.970 | 2.400 |
Rmerge | 0.167 | 0.097 | 0.554 |
Rmeas | 0.187 | 0.108 | 0.629 |
Rpim | 0.082 | 0.045 | 0.292 |
Total number of observations | 169376 | 3364 | 16172 |
Number of reflections | 34103 | 693 | 3538 |
<I/σ(I)> | 5.5 | 8.8 | 2.1 |
Completeness [%] | 99.8 | 99 | 98.7 |
Redundancy | 5 | 4.9 | 4.6 |
CC(1/2) | 0.977 | 0.985 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | (0.2 M calcium chloride and 20% [w/v] PEG3350) |