8EN0
Structure of GII.17 norovirus in complex with Nanobody 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 74.820, 85.459, 223.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.960 - 2.990 |
R-factor | 0.2521 |
Rwork | 0.249 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f4o |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 111.926 | 111.926 | 3.170 |
High resolution limit [Å] | 2.990 | 8.940 | 2.990 |
Rmerge | 0.286 | 0.054 | 1.859 |
Rmeas | 0.298 | 0.057 | 1.934 |
Total number of observations | 188769 | ||
Number of reflections | 14280 | 630 | 2314 |
<I/σ(I)> | 9.1 | 33.92 | 1.31 |
Completeness [%] | 95.5 | 99.5 | 97.9 |
Redundancy | 13.219 | 12.16 | 13.34 |
CC(1/2) | 0.994 | 0.994 | 0.566 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.8 M ammonium sulfate and 0.1M citric acid [pH 4] |