8EMZ
Structure of GII.17 norovirus in complex with Nanobody 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.961, 90.545, 59.397 |
Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
Resolution | 48.850 - 1.400 |
R-factor | 0.1959 |
Rwork | 0.195 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f4o |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 117.530 | 4.190 |
High resolution limit [Å] | 1.400 | 2.970 |
Number of reflections | 85355 | 2485 |
<I/σ(I)> | 19.1 | |
Completeness [%] | 98.5 | |
Redundancy | 7.009 | 7.015 |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M bicine [pH 9] and 30% [w/v] PEG6000 |