8EMI
Crystal structure of a HLA-B*35:01-NP8 epitope from 2005 H1N1 influenza strain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.869, 81.528, 109.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.228 - 1.573 |
R-factor | 0.177453306722 |
Rwork | 0.176 |
R-free | 0.20769 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lks |
RMSD bond length | 0.014 |
RMSD bond angle | 1.495 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.300 | 1.630 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmeas | 0.042 | 0.394 |
Number of reflections | 63452 | 6187 |
<I/σ(I)> | 20.9 | 2.82 |
Completeness [%] | 99.0 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 297 | PEG |