8EMG

Crystal structure of a HLA-B*35:01-NP7 epitope from 2002 H2N1 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	51.167, 81.925, 110.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.509 - 1.830
R-factor	0.170824965487
Rwork	0.169
R-free	0.20559
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.010
RMSD bond angle	1.488
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.510	1.900
High resolution limit [Å]	1.830	1.830
Number of reflections	41590	4007
<I/σ(I)>	15.7
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG