8EMC
CryoEM characterization of BrxL -- a unique AAA+ phage restriction Factor.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-10 (5k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 96079 |
| Software | cryoSPARC,SerialEM,UCSF Chimera,Coot |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING ONLY |
