8ELR
HRAS R97F Crystal Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-09-16 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 39.258, 39.258, 157.984 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.000 - 2.050 |
Rwork | 0.188 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ctq |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.18.2) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
Rmerge | 0.051 | 0.029 | 0.291 |
Rmeas | 0.054 | 0.031 | 0.315 |
Rpim | 0.018 | 0.011 | 0.119 |
Total number of observations | 86621 | ||
Number of reflections | 9528 | 568 | 469 |
<I/σ(I)> | 11.3 | ||
Completeness [%] | 99.3 | 99.6 | 100 |
Redundancy | 9.1 | 8.3 | 6.8 |
CC(1/2) | 1.000 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Calcium Acetate PEG3350, Magnesium Chloride, HEPES, Sodium Chloride, DTT |