8ELN
Crystal Structure of Nanobody VHH222 Bound to Its Antigen PA14 Cif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.222, 164.326, 107.291 |
Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.161 | 1.013 |
Rmeas | 0.190 | 1.188 |
Rpim | 0.100 | 0.617 |
Number of reflections | 101735 | 10117 |
<I/σ(I)> | 6.88 | 1.38 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.988 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292.8 | 20% (w/v) PEG4000, 200 mM ammonium acetate, 100 mM sodium acetate, pH 5 |