8ELL
Apo human biliverdin reductase beta (cryogenic)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE 7B2 |
Synchrotron site | CHESS |
Beamline | 7B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.12710 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.187, 45.668, 104.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.100 - 1.520 |
R-factor | 0.1609 |
Rwork | 0.160 |
R-free | 0.18750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6opl |
RMSD bond length | 0.008 |
RMSD bond angle | 0.975 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.100 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.115 | 1.027 |
Rmeas | 0.120 | 1.085 |
Rpim | 0.034 | 0.338 |
Number of reflections | 31069 | 2891 |
<I/σ(I)> | 12 | 1.4 |
Completeness [%] | 97.8 | 92.5 |
Redundancy | 12.5 | 10.1 |
CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 1-4% PEG 20000. Equilibrated over 0.25-0.5 M NaCl. BLVRB 18 mg/mL. |