8EL4

Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 in a tight interface filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	63.529, 71.258, 48.283
Unit cell angles	90.00, 108.47, 90.00

Refinement procedure

Resolution	45.800 - 1.730
R-factor	0.1902
Rwork	0.188
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmx
RMSD bond length	0.013
RMSD bond angle	1.415
Data reduction software	DIALS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.135
Number of reflections	42700	2325
<I/σ(I)>	8.2	0.7
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.997	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	PEG3350 25% (w/v) + 0.01M Sarcosine, 150 nL mother + 150 nL protein solution, Cryoprotectant of 20% Gylcerol