8EKV

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGCGGATGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977410
Spacegroup name	P 1 21 1
Unit cell lengths	42.616, 60.600, 44.483
Unit cell angles	90.00, 116.20, 90.00

Refinement procedure

Resolution	33.330 - 1.620
R-factor	0.1665
Rwork	0.164
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.011
RMSD bond angle	1.348
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.330	1.678
High resolution limit [Å]	1.620	1.620
Rmerge	0.034	0.332
Rmeas	0.039	0.384
Rpim	0.020	0.190
Number of reflections	25338	2511
<I/σ(I)>	22.65	4.04
Completeness [%]	97.6	96.64
Redundancy	3.8	4
CC(1/2)	0.997	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350