8EKL
Clostridioides difficile binary toxin translocase CDTb wild-type after calcium depletion from receptor binding domain 1 (RBD1) - Class 1
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.06 |
| Number of Particle | 1109860 |
| Software | cryoSPARC,PHENIX,SerialEM,UCSF Chimera,Coot |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
