8EKJ
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAATGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977410 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.720, 60.027, 44.627 |
Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
Resolution | 33.300 - 1.540 |
R-factor | 0.1663 |
Rwork | 0.165 |
R-free | 0.19180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.010 |
RMSD bond angle | 1.259 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 1.595 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.039 | 0.390 |
Rmeas | 0.046 | 0.457 |
Rpim | 0.024 | 0.235 |
Number of reflections | 29862 | 2945 |
<I/σ(I)> | 17.49 | 3.05 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.999 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, 2% PEG 3350 |