8EKJ

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAATGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977410
Spacegroup name	P 1 21 1
Unit cell lengths	42.720, 60.027, 44.627
Unit cell angles	90.00, 116.23, 90.00

Refinement procedure

Resolution	33.300 - 1.540
R-factor	0.1663
Rwork	0.165
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.010
RMSD bond angle	1.259
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	1.595
High resolution limit [Å]	1.540	1.540
Rmerge	0.039	0.390
Rmeas	0.046	0.457
Rpim	0.024	0.235
Number of reflections	29862	2945
<I/σ(I)>	17.49	3.05
Completeness [%]	99.6	99.9
Redundancy	3.7	3.6
CC(1/2)	0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, 2% PEG 3350