8EJV
The crystal structure of Pseudomonas putida PcaR in complex with succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | .925 |
| Spacegroup name | P 62 |
| Unit cell lengths | 148.822, 148.822, 56.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.210 - 2.430 |
| R-factor | 0.2107 |
| Rwork | 0.208 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ia2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.813 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.210 | 2.540 |
| High resolution limit [Å] | 2.430 | 2.500 |
| Rmeas | 0.071 | 0.628 |
| Rpim | 0.022 | 0.193 |
| Number of reflections | 26706 | 1243 |
| <I/σ(I)> | 56.16 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.3 | |
| CC(1/2) | 1.000 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Reservoir: 1.8 M ammonium phosphate monophosphate, 0.1 M succinic acid, pH 7, 1.25% w/v Benzyltriethylammonium chloride |
