8EJO

Crystal structure of the homeodomain of Platypus sDUX in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	74.022, 74.022, 97.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.910 - 2.670
R-factor	0.225
Rwork	0.224
R-free	0.23750
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.020	74.020	2.800
High resolution limit [Å]	2.670	8.840	2.670
Rmerge	0.059	0.033	1.253
Rmeas	0.064	0.036	1.366
Rpim	0.024	0.014	0.531
Total number of observations	50642	1363	6516
Number of reflections	8096	261	1021
<I/σ(I)>	14.3	35.9	1
Completeness [%]	99.2	96.9	98
Redundancy	6.3	5.2	6.4
CC(1/2)	0.999	0.999	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M CaCl2, 0.05M HEPES sodium 7.5, 28 % v/v PEG 400, 0.002M Spermine