8EIN
Crystal structure of WT cyanophycin dipeptide hydrolase CphZ from Acinetobacter baylyi DSM587
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-04 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.632, 128.015, 107.078 |
Unit cell angles | 90.00, 129.48, 90.00 |
Refinement procedure
Resolution | 48.940 - 2.700 |
R-factor | 0.2433 |
Rwork | 0.242 |
R-free | 0.26220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ab initio model generated with Rosetta |
RMSD bond length | 0.014 |
RMSD bond angle | 1.772 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.800 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.037 | |
Number of reflections | 39871 | 1138 |
<I/σ(I)> | 15.26 | |
Completeness [%] | 83.8 | |
Redundancy | 5.3 | |
CC(1/2) | 0.998 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M bis-tris propane pH 7.5, 24% PEG3350, 0.2 M NaBr, 10 mM spermine and 10 mM LiCl |