8EIL
C-Terminal Domain of BrxL from Acinetobacter BREX type I phage restriction system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9774 |
Spacegroup name | F 2 3 |
Unit cell lengths | 213.344, 213.344, 213.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.940 - 2.250 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold2 model |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 (v722) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 6.100 | 2.250 |
Rmerge | 0.159 | 0.059 | 2.636 |
Rmeas | 0.161 | 0.060 | 2.679 |
Rpim | 0.025 | 0.010 | 0.476 |
Total number of observations | 1513126 | ||
Number of reflections | 37994 | 1986 | 1878 |
<I/σ(I)> | 2.9 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 39.8 | 39.9 | 31.3 |
CC(1/2) | 1.000 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 45% Tacsimate |