8EIB

Crystal structure of beta-catenin and the MDM2 p53-binding domain in complex with H329, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 21 21
Unit cell lengths	55.620, 69.830, 165.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.170 - 3.760
R-factor	0.2671
Rwork	0.264
R-free	0.29810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uwi
RMSD bond length	0.002
RMSD bond angle	0.614
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.170	4.200
High resolution limit [Å]	3.760	3.760
Number of reflections	6955	1935
<I/σ(I)>	5.1	2.7
Completeness [%]	99.3
Redundancy	12.2
CC(1/2)	0.972	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Calcium acetate pH 7.5, 20% w/v PEG 3350