8EI9

Crystal structure of beta-catenin and the MDM2 p53-binding domain in complex with H332, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	87.035, 95.318, 166.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 3.900
R-factor	0.2668
Rwork	0.265
R-free	0.31540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uwi
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	4.360
High resolution limit [Å]	3.900	3.900
Number of reflections	13180	3642
<I/σ(I)>	3.2
Completeness [%]	99.8
Redundancy	5.8
CC(1/2)	0.923	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES Sodium Salt pH6.5, 10% v/v 2-Propanol, 25% v/v PEG 400