8EI2
Crystal structure of the N-terminal domain of CUL5 in complex with H314, a Helicon Polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 6 |
Unit cell lengths | 173.030, 173.030, 57.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.880 - 2.800 |
R-factor | 0.3398 |
Rwork | 0.339 |
R-free | 0.35750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wzk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.880 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.322 | |
Number of reflections | 24154 | 3518 |
<I/σ(I)> | 5 | 1.8 |
Completeness [%] | 98.2 | 99.3 |
Redundancy | 13.2 | 13.7 |
CC(1/2) | 0.988 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% w/v PEG 8000, 0.1M MES Sodium Salt pH6.5, 0.2M Ammonium Sulfate, 4% v/v 1,3-Propanediol |