8EI1
Crystal structure of the N-terminal domain of CUL4B in complex with H316, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.650, 99.650, 366.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.820 - 2.890 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a64 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.360 | 3.000 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.110 | |
| Number of reflections | 42685 | 4410 |
| <I/σ(I)> | 23.9 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.6 | 27.6 |
| CC(1/2) | 1.000 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30% w/v PEG 4000 |
