8EHZ

Crystal structure of the STUB1 TPR domain in complex with H317, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 32
Unit cell lengths	63.209, 63.209, 69.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.080 - 2.060
R-factor	0.2439
Rwork	0.241
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nsv
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.080	2.120
High resolution limit [Å]	2.060	2.060
Rmerge	0.078	0.796
Number of reflections	19293	1471
<I/σ(I)>	15.2	2.6
Completeness [%]	100.0	100
Redundancy	10.5	10.6
CC(1/2)	0.998	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1M Sodium acetate, 0.1M sodium citrate pH6.0, 10% PEG4000