8EHT
Cryo-EM reconstruction of the CS20 bacterial adhesion pili
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.4 |
| Number of Particle | 9590890 |
| Software | RELION,PHENIX,ISOLDE,Leginon,CTFFIND,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
