8EHD
Structure of Tannerella forsythia potempin E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.920, 36.560, 37.160 |
| Unit cell angles | 90.00, 105.64, 90.00 |
Refinement procedure
| Resolution | 35.780 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ehc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.492 |
| Rmeas | 0.057 | 0.560 |
| Number of reflections | 10402 | 1541 |
| <I/σ(I)> | 21.2 | 3.2 |
| Completeness [%] | 98.1 | 91.4 |
| Redundancy | 6.1 | 4.4 |
| CC(1/2) | 1.000 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Native PotE at 9.2 mg/mL in 50 mM sodium chloride, 5 mM Tris-HCl pH 8.0 was crystallised from 20% (w/v) PEG 8,000, 100 mM Hepes pH 7.5 |
