8EGX

Complex of Fat4(EC1-4) bound to Dchs1(EC1-4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-03
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.222, 82.102, 224.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 3.688
R-factor	0.2722
Rwork	0.271
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8egw 6vft
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	3.820
High resolution limit [Å]	3.688	3.688
Number of reflections	12968	1214
<I/σ(I)>	4.05
Completeness [%]	95.9
Redundancy	2
CC(1/2)	0.994	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M MES, pH 6.5; 0.2 M Sodium Chloride; 10% PEG 4000