8EFZ
Crystal structure of CcNikZ-II, apoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-01-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.606, 110.942, 73.686 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 29.710 - 2.380 |
| R-factor | 0.2284 |
| Rwork | 0.222 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oet |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.545 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.101 | 0.536 |
| Rpim | 0.057 | 0.267 |
| Number of reflections | 40340 | 1752 |
| <I/σ(I)> | 20.66 | 1.64 |
| Completeness [%] | 93.9 | 81.6 |
| Redundancy | 4.2 | 3.4 |
| CC(1/2) | 0.990 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 30 mg/mL protein and reservoir solution 0.1 M Hepes pH 7.5, 30% (w/v) polyethylene glycol 1000 |
