8EFZ

Crystal structure of CcNikZ-II, apoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-01-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	73.606, 110.942, 73.686
Unit cell angles	90.00, 113.72, 90.00

Refinement procedure

Resolution	29.710 - 2.380
R-factor	0.2284
Rwork	0.222
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oet
RMSD bond length	0.002
RMSD bond angle	0.545
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MrBUMP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.101	0.536
Rpim	0.057	0.267
Number of reflections	40340	1752
<I/σ(I)>	20.66	1.64
Completeness [%]	93.9	81.6
Redundancy	4.2	3.4
CC(1/2)	0.990	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	30 mg/mL protein and reservoir solution 0.1 M Hepes pH 7.5, 30% (w/v) polyethylene glycol 1000