8EFW

Structure of SdeA DUB Domain disulfide crosslinked with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	90.905, 90.905, 65.821
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.360 - 2.810
Rwork	0.242
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 5crb
RMSD bond length	0.008
RMSD bond angle	1.501
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.450	2.910
High resolution limit [Å]	2.810	2.810
Number of reflections	7850	7829
<I/σ(I)>	11.9
Completeness [%]	98.8
Redundancy	2.9
CC(1/2)	0.995	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	2.8M sodium acetate: HCl pH7