8EFM

Structure of coral STING receptor from Stylophora pistillata in complex with 2',3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	89.765, 89.765, 174.778
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.870 - 2.130
R-factor	0.1999
Rwork	0.197
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.870	2.200
High resolution limit [Å]	2.130	2.140
Number of reflections	23858	23858
<I/σ(I)>	14.6
Completeness [%]	99.8	98.1
Redundancy	13.6	8.9
CC(1/2)	0.998	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	28% PEG 5000 MME, 100 mM Tris-HCl pH 8.6, 200 mM lithium sulfate