8EFM
Structure of coral STING receptor from Stylophora pistillata in complex with 2',3'-cGAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 89.765, 89.765, 174.778 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.870 - 2.130 |
R-factor | 0.1999 |
Rwork | 0.197 |
R-free | 0.22650 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.532 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.870 | 2.200 |
High resolution limit [Å] | 2.130 | 2.140 |
Number of reflections | 23858 | 23858 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 99.8 | 98.1 |
Redundancy | 13.6 | 8.9 |
CC(1/2) | 0.998 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 28% PEG 5000 MME, 100 mM Tris-HCl pH 8.6, 200 mM lithium sulfate |