8EF1

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ103-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	71.480, 80.123, 175.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.829 - 1.870
R-factor	0.1741
Rwork	0.173
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqq
RMSD bond length	0.004
RMSD bond angle	0.751
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	1.970
High resolution limit [Å]	1.870	1.870
Rmerge	0.050	0.430
Number of reflections	37647	5536
<I/σ(I)>	11.9
Completeness [%]	90.5	92.1
Redundancy	2.4
CC(1/2)	0.990	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20.0% PEG 4K, 0.2M NaAc, 0.1M Na Citrate (pH5.6)