8EF0

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ104-D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 32 2 1
Unit cell lengths	71.728, 71.728, 159.991
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.947 - 2.550
R-factor	0.1876
Rwork	0.185
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqq
RMSD bond length	0.005
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.480	2.480
Number of reflections	17381	1540
<I/σ(I)>	9.2
Completeness [%]	98.4	89.1
Redundancy	5.9
CC(1/2)	0.810	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	26% PEG 8K, 0.2M ZnAc, 0.1M Tris-HCl (pH8.5)