8EEZ
Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ100-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.981, 71.226, 114.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.896 - 2.250 |
R-factor | 0.1879 |
Rwork | 0.185 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fqq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.196 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 22274 | 1968 |
<I/σ(I)> | 7.6 | |
Completeness [%] | 92.5 | |
Redundancy | 4.1 | |
CC(1/2) | 0.990 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 22.5% PEG 4K, 22.5% IPA, 0.1M Na Citrate (pH5.6) |