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8EEZ

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ100-C

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyPIXEL
Collection date2017-08-21
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.97
Spacegroup nameP 21 21 21
Unit cell lengths55.981, 71.226, 114.176
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution14.896 - 2.250
R-factor0.1879
Rwork0.185
R-free0.23280
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4fqq
RMSD bond length0.009
RMSD bond angle1.196
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX (1.11.1_2575)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.260
High resolution limit [Å]2.1902.190
Number of reflections222741968
<I/σ(I)>7.6
Completeness [%]92.5
Redundancy4.1
CC(1/2)0.9900.579
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29122.5% PEG 4K, 22.5% IPA, 0.1M Na Citrate (pH5.6)

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