8EEN
C. ammoniagenes monoamine oxidase (MAO) C424S variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.229, 117.835, 141.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 1.460 |
| R-factor | 0.1856 |
| Rwork | 0.185 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.830 | 1.514 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.096 | 0.562 |
| Rmeas | 0.105 | 0.651 |
| Rpim | 0.041 | 0.998 |
| Number of reflections | 173307 | 16432 |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 99.5 | 95.37 |
| Redundancy | 6.4 | 3.9 |
| CC(1/2) | 0.998 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 10 - 15% w/v PEG-3350, 0.1 M sodium malonate, pH 5.0 |
