Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2022-07-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.243, 70.243, 287.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.230 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 model for UNC-6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 2.164 |
| Rmeas | 0.090 | 2.245 |
| Number of reflections | 25806 | 4008 |
| <I/σ(I)> | 19.43 | 1.25 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 13.1 | 13.7 |
| CC(1/2) | 0.999 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris, pH 8.0, 10% (w/v) Polyethylene glycol 8,000 |
