8ED1
Bovine Fab 5C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.901, 197.413, 70.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.380 - 2.310 |
| R-factor | 0.2364 |
| Rwork | 0.235 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6oo0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 2.340 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.151 | 0.841 |
| Rmeas | 0.159 | 0.902 |
| Rpim | 0.047 | 0.318 |
| Number of reflections | 27526 | 8370 |
| <I/σ(I)> | 15.8 | 2.1 |
| Completeness [%] | 90.6 | 75.2 |
| Redundancy | 9.9 | 7.4 |
| CC(1/2) | 0.989 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 0.1M Tris, 10% glycerol, 20% Peg300, 5% Peg8000 |
