8EBC
Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Listeria monocytogenes in the complex with IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-12 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.977, 95.699, 97.604 |
| Unit cell angles | 111.36, 105.17, 107.61 |
Refinement procedure
| Resolution | 39.880 - 2.500 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 1PVN |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.427 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.426 |
| Rpim | 0.051 | |
| Number of reflections | 78331 | 3560 |
| <I/σ(I)> | 13.11 | 1.71 |
| Completeness [%] | 97.7 | 88.6 |
| Redundancy | 2.2 | 2 |
| CC(1/2) | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 289 | 0.2 M ammonium formate pH 6.6, 20 % (w/v) PEG |
